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reference r d systems human il 2 quantikine elisa kit  (R&D Systems)


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    R&D Systems reference r d systems human il 2 quantikine elisa kit
    Reference R D Systems Human Il 2 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+2+r+d+systems/Human+IL-2+Quantikine+ELISA+Kit/us12611429-3333-0-1
    Average 95 stars, based on 159 article reviews
    reference r d systems human il 2 quantikine elisa kit - by Bioz Stars, 2026-09
    95/100 stars

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    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies phospho-NF-kB p65 (Ser536) Cell Signaling Technology Cat# 3033S; RRID: AB_331284 NF-kB p65 Cell Signaling Technology Cat# 8242S; RRID: AB_10859369 b-Actin Cell Signaling Technology Cat# 3700S; RRID: AB_2242334 MYD88 Cell Signaling Technology Cat# 4283S; RRID: AB_10547882 LYN Cell Signaling Technology Cat# 2796S; RRID: AB_2138391 Lamin-B1 Santa Cruz Cat# sc-374015; RRID: AB_10947408 MYD88 Santa Cruz Cat# sc-74532; RRID: AB_1126429 TLR4 Santa Cruz Cat# sc-293072; AB_10611320 LYN Santa Cruz Cat# sc-7274; AB_627897 Normal mouse IgG Santa Cruz Cat# sc-2025; AB_737182 CD36 Abcam Cat# ab133625; RRID: AB_2716564 ZDHHC6 Abcam Cat# ab121423; RRID: AB_11129943 CD3 Abcam Cat# ab135372; RRID: AB_2884903 TLR4 CUSABIO Technology Cat# CSB-PA001434 InVivoPlus anti-mouse NK1.1 BioXcell Cat# BE0036; RRID: AB_1107737 InVivoMab anti-mouse CD8b BioXcell Cat# BE0223; RRID: AB_2687706 anti-mouse CD4 BioXcell Cat# BP0003-1; RRID: AB_1107636 InVivoPlus anti-mouse PD-L1 BioXcell Cat# BP0101; RRID: AB_10949073 InVivoPlus mouse IgG2a BioXcell Cat# BE0085; RRID: AB_1107771 InVivoPlus rat IgG1 BioXcell Cat# BE0088; RRID: AB_1107775 InVivoPlus rat IgG2b BioXcell Cat# BP0090; RRID: AB_1107780 Phospho-NFkB p65 (Ser529) Antibody, PE Invitrogen Cat# MA5-37165; RRID: AB_2897099 Arginase 1 Antibody, APC Invitrogen Cat# 17-3697-80; RRID: AB_2734834 Anti-human PD-L1-PE-Cy7 Invitrogen Cat# 25-5983-41; RRID: AB_1907369 Anti-mouse PD-1-BV785 BioLegend Cat# 135225; RRID: AB_2563680 Anti-mouse LAG-3-BV785 BioLegend Cat# 125219; RRID: AB_2566571 Anti-human PD-1-PE-Cy7 BioLegend Cat# 329917; RRID: AB_2159325 Anti-human CD8-FITC BioLegend Cat# 344704; RRID: AB_1877178 Anti-human CD14-BV421 BioLegend Cat# 301830; RRID: AB_10959324 Anti-mouse TIM3-PE eBioscience Cat# 12-5870-82; RRID: AB_465974 OVA257-264 (SIINFEKL) peptide bound to H-2K b Antibody, APC eBioscience Cat# 17-5743-80; RRID: AB_1311288 Anti-mouse CD36-APC eBioscience Cat# 17-0362-82; RRID: AB_2734967 Anti-mouse PD-L1-PE-Cy7 eBioscience Cat# 25-5982-82; RRID: AB_2573509 Anti-human TLR4-APC eBioscience Cat# 17-9917-41; RRID: AB_2016622 Anti-mouse CD107a-BV421 BD Biosciences Cat# 564347; RRID: AB_2738760 Anti-human CD33-BV421 BD Biosciences Cat# 562854; RRID: AB_2737405 Anti-human CD36-APC BD Biosciences Cat# 561822; RRID: AB_398480 Anti-human CD36-FITC BD Biosciences Cat# 555454; RRID: AB_2291112 Anti-human CD34-FITC BD Biosciences Cat# 555821; RRID: AB_396150 Anti-human CD34-APC BD Biosciences Cat# 561209; RRID: AB_10683161 Anti-human CD45-APC-Cy7 BD Biosciences Cat# 557833; RRID: AB_396891 Anti-human CD56-PE-Cy5 BD Biosciences Cat# 555517; RRID: AB_395907 (Continued on next page) Cell Reports Medicine 5, 101592, June 18, 2024 e1 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Human IL-3 PEPROTECH Cat# 200-03 Human IL-6 PEPROTECH Cat# 200-06 Human SCF PEPROTECH Cat# 300-07 Human TPO PEPROTECH Cat# 300-18 Human IL-2 R&D Systems Cat# BT-002 Mouse IL-2 R&D Systems Cat# 402-ML APC-conjugated H-2Kb OVA Tetramer-SIINFEKL MBL Cat# TS-5001-2C Protease inhibitor cocktail Selleck Cat# B14001 Phosphatase inhibitor cocktail Selleck Cat# B15001 Sulfosuccinimidyl oleate MedChemExpress Cat# HY-112847 SC75741 MedChemExpress Cat# HY-10496 Fluvastatin MedChemExpress Cat# HY-14664 C75 MedChemExpress Cat# HY-12364 Bezafibrate MedChemExpress Cat# HY-B0637 Etomoxir MedChemExpress Cat# HY-50202 ABT-737 MedChemExpress Cat# HY-50907 Cytarabine MedChemExpress Cat# HY-13605 Coumermycin A1 MedChemExpress Cat# HY-N7452 Novobiocin MedChemExpress Cat# HY-B0425A TAK-242 MedChemExpress Cat# HY-11109 PP1 MedChemExpress Cat# HY-13804 Decitabine MedChemExpress Cat# HY-A0004 a-Tocopherol MedChemExpress Cat# HY-N0683 Busulfan MedChemExpress Cat# HY-B0245 Nor-NOHA ApexBio Technology Cat# C5407 OxLDL Solarbio Cat# IL2150 LDL Solarbio Cat# IL2110 HDL Solarbio Cat# IL2130 Palmitate Cayman Chemical Cat# 10006627 Oleic acid Cayman Chemical Cat# 90260 Arachidonic Acid Cayman Chemical Cat# 90010 CFSE Biolegend Cat# 423801 SIINFEKL peptide Nanjing Peptide Biotech N/A alkynyl palmitate click chemistry tools Cat# 1165 Critical commercial assays StemSpan SFEM II STEMCELL Technologies Cat# 09605_C EasySep Human Cord Blood CD34 Positive Selection Kit III STEMCELL Technologies Cat# 17897 (Continued on next page) e2 Cell Reports Medicine 5, 101592, June 18, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein Enrichment Kit for capture of alkynemodified proteins click chemistry tools Cat# 1441 Fatty Acid Uptake Assay Kit Abnova Cat# KA4084 Oxidized LDL Uptake Assay Kit Cayman Chemical Cat# 601180 Nuclear and Cytoplasmic Protein Extraction Kit Sangon Biotech Cat# C510001 Pan T cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-535 Pan T cell Isolation Kit II, mouse Miltenyi Biotec Cat# 130-095-130 Mouse CD8+ T cell Isolation Kit STEMCELL Technologies Cat# 19853 T cell Activation/Expansion Kit, mouse Miltenyi Biotec Cat# 130-093-627 T cell Activation/Expansion Kit, human Miltenyi Biotec Cat# 130-091-441 Deposited data ChIP-seq data of p65 Lin et al.45 GSE103477 RNA-seq data of CD36KO vs. CD36NC MV4-11 cells This paper GSE209783 RNA-seq data of decitabine-treated vs. untreated THP-1 cells This paper GSE200510 RNA-seq data of decitabine-treated vs. untreated MV4-11 cells This paper GSE225384 RNA-seq data of AML patients Li et al.34 GSE37642 Experimental models: Cell lines MV4-11 ATCC Cat# CRL-9591 HEK-293T ATCC Cat# CRL-3216 MOLM-13 DSMZ Cat# ACC 544 OCIAML2 DSMZ Cat# ACC 99 OCIAML3 DSMZ Cat# ACC 582 NOMO1 DSMZ Cat# ACC 542 SET2 DSMZ Cat# ACC 608 U937 ATCC Cat# CRL-1593.2 C1498 ATCC Cat# TIB-49 THP-1 ATCC Cat# TIB-202 NB4 DSMZ Cat# ACC 207 K-562 ATCC Cat# CCL-243 Experimental models: Organisms/strains NOD.Cg-PrkdcscidIl2rgtm1Sug/JicCrl (NOG) mice Vital River Cat# 408 C57BL/6JNifdc mice Vital River Cat# 219 NOD.Cg-PrkdcscidIl2rgtm1SugB2mem1TacH2-Ab1tm1Doi/ JicCrl (NOG-dKO) mice Vital River Cat# 411 Oligonucleotides shRNA sequences This paper Table S4 sgRNA sequences This paper Table S4 primers used for RT-PCR This paper Table S4 primers used for NGS-BSP This paper Table S4 Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 LentiGuide Puro-P2A-EGFP Addgene Cat# 137729 lentiCas9-Blast Addgene Cat# 52962 pLKO.5 Sigma-Aldrich Cat# SHC201 pLV-EF1a-IRES-Neo Addgene Cat# 85139 (Continued on next page) Cell Reports Medicine 5, 101592, June 18, 2024 e3 Article ll OPEN ACCESS

    Article Title: GATA1-mediated Notch signaling augment antitumor immunity of CD11b + CD27 - natural killer cells maturation via BCL9/β-catenin signal.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse-CD45-PerCP/Cy5.5 Invitrogen Cat#45-0451-82; RRID:AB_1107002 Anti-mouse-CD3e-eFluor 450 Invitrogen Cat#48-0031-80; RRID:AB_10733280 Anti-human/mouse-Granzyme B-Alexa Fluor 700 Biolegend Cat#372222; RRID:AB_2728389 Anti-mouse-Granzyme B-eFluor 450 eBioscience Cat#48-8898-82; RRID:AB_11149362 Anti-mouse-CD335 (NKp46)-Brilliant Violet 605 Biolegend Cat#137619; RRID:AB_2562452 Anti-mouse-CD335 (NKp46)-FITC eBioscience Cat#11-3351-82; RRID:AB_1210843 Anti-mouse-CD314 (NKG2D)-Alexa Fluor 488 eBioscience Cat#53-5882-82; RRID:AB_2811867 Anti-mouse-NK1.1-APC BioLegend Cat#108709; RRID:AB_313396 Anti-mouse-CD27-PE Biolegend Cat#124210; RRID:AB_1236459 Anti-mouse-CD11b-PE/Cy7 Biolegend Cat#10216 Anti-human-CD16-PE BioLegend Cat#360704; RRID:AB_2562749 Anti-human-CD56-APC BioLegend Cat#362504; RRID:AB_2563913 Anti-mouse-Ki-67-PE eBioscience Cat#12-5698-80; RRID:AB_11149672 Anti-human -CD3e-FITC MULTISCIENCES Cat#AH00301-100; RRID:AB_2890114 Anti-mouse -CD3e-FITC MULTISCIENCES Cat#AM003E0201-250 NOTCH1 Polyclonal antibody Proteintech Cat#20687-1-AP; RRID:AB_10700012 β-Actin Proteintech Cat#66009-1-Ig; RRID:AB_2687938 Bacterial and virus strains HBAAV2/9-ZsGreen Hanbio Cat#75010302 HBAAV2/9-Klrb1c-m-cre-3xflag-ZsGreen Hanbio Cat#81071807 Cignal Reporter Assay Kits QIAGEN Cat# CCS018L Chemicals, peptides, and recombinant proteins AG RNAex Pro Reagent Accurate Biology Cat#AG21102 Recombinant Mouse IL-2 R&D Systems Cat#402-ML-020 Recombinant Human IL-2 R&D Systems Cat#202-IL-050 DAPT APExBIO Cat#A8200 hsBCL9Z474 This paper N/A DMSO SIGMA Cat#D2660-100ML Ficoll-PaqueTM cytiva Cat#17144002 Fixation/Perm Diluent eBioscience Cat#00-5223-56 Fixation/Permeabilization Concentrate eBioscience Cat#00-5123-43 Permeabilization Buffer (10X) eBioscience Cat#00-8333-56 IC Fixation Buffer eBioscience Cat#00-8222-49 Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) eBioscience Cat#00-4975-03 ICG-001 APExBIO Cat#A8217 PageRulerTM Plus Prestained Protein Ladder Thermo Fisher Scientific Cat#26619 Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Retronectin Takara Cat#T100A FuGENE® HD Transfection Reagent Promega Cat#E2312 (Continued on next page) 18 Cell Reports 44, 115708, May 27, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays EasySepTM Mouse NK Cell Isolation Kit STEMCELL Cat#19855 EasySepTM Human NK Cell Isolation Kit STEMCELL Cat# 17955 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biology Cat#AG11701 Evo M-MLV RT Premix for qPCR Accurate Biology Cat#AG11706 ChIP Assay Kit Beyotime Cat#P2078 PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat#23225 Cytotoxicity Assay Kit (CFSE, 7-AAD) Abcam Cat#ab270780 PAGE Gel Fast Preparation Kit Epizyme Biotech Cat#PG111 Dual-LumiTM Luciferase Reporter Gene Assay Kit Beyotime Biotechnology Cat#RG088S Cell Counting Kit-8 Meilunbio Cat#MA0218-2 Deposited data scRNA-seq data This paper https://doi.org/10.6084/m9.figshare.

    Article Title: A T-cell-based immunogenicity protocol for evaluating human antigen-specific responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC mouse anti-human CD3 BioLegend Clone: SK7, Cat# 344803 APC mouse anti-human CD8 BioLegend Clone: RPA-T8, Cat# 301049 Brilliant Violet 785 mouse anti-human CD4 BioLegend Clone: RPA-T4, Cat# 300554 PE mouse anti-human IFN-g BioLegend Clone: B27, Cat# 506507 PE/Cyanine7 mouse anti-human TNF-a BioLegend Clone: Mab11, Cat# 502930 Brilliant Violet 605 rat anti-human IL-2 BioLegend Clone: MQ1-17H12, Cat# 500332 Purified mouse anti-human CD28 BD Biosciences Clone: CD28.2, Cat# 555726 Purified mouse anti-human CD49d BD Biosciences Clone: 9F10, Cat# 555502 Biological samples Healthy human peripheral blood mononuclear cells New York Blood Center https://nybloodcenter.org/ Chemicals, peptides, and recombinant proteins Custom peptides GenScript https://www.genscript.com/ peptide.html PepMix Human (MOG) JPT PM-MOG CEFT pool JPT PM-CEFT-3 (Continued on next page) 2 STAR Protocols 2, 100758, September 17, 2021 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Human granulocyte-macrophage colony-stimulating factor (GM-CSF) (LEUKINE (sargramostim)) Sanofi NDC 0024-5843-05 Human IL-4 R&D Systems Cat# 204-IL-010 Human Flt-3 Ligand R&D Systems Cat# 308-FKN-025 R848 InvivoGen Cat# tlrl-r848-5 Ultrapure LPS, Salmonella minnesota InvivoGen Cat# tlrl-smlps Human IL-1 beta R&D Systems Cat# 201-LB-010 Human IL-2 R&D Systems Cat# 202-IL-050 Human IL-7 R&D Systems Cat# 207-IL-025 Human IL-15 PeproTech Cat# 200-15 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# 79346-1MG Ionomycin calcium salt Sigma-Aldrich Cat# I0634-1MG Software and algorithms FACSDiva BD Biosciences N/A FlowJo v10.7.1 Tree Star www.flowjo.com/ Prism v9.1.0 GraphPad www.graphpad.com/ Other UltraComp eBead Plus Compensation Beads Thermo Fischer Scientific Cat# 01-3333-41 ArC Amine Reactive Compensation Bead Kit Thermo Fischer Scientific Cat# A10346 LIVE/DEAD Fixable Blue Dead Cell Stain Kit Thermo Fischer Scientific Cat# L34962 Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 GolgiStop: Protein Transport Inhibitor (containing Monensin) BD Biosciences Cat# 554724 GolgiPlug: Protein Transport Inhibitor (containing Brefeldin A) BD Biosciences Cat# 555029 Deoxyribonuclease I from bovine pancreas Sigma-Aldrich Cat# D4513-1VL DPBS, calcium, magnesium Gibco Cat# 14040133 X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza Cat# 04-418Q RPMI 1640 Medium Gibco Cat# 11875-093 Heat inactivated human serum Gemini Bio-Products Cat# 100-512 Nalgene Rapid-Flow Sterile Single Use Vacuum Filter Units Thermo Fischer Scientific Cat# 566-0020 96-well Clear Round Bottom TC-treated Microplate Corning Cat# 3799 ll OPEN ACCESSProtocol ..

    Article Title: Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance.
    Article Snippet: In brief Classic tumor cell apoptosis and pyroptosis induced by tumor-specific T cells may not trigger sufficient primary responses and prevent tumor antigen escape-mediated acquired resistance.. Zheng et al. uncover a special tumor cell death induced by CAR-T, autosis, which has not been attributed to any T cell killingmechanism before but canmediate a potent type of bystander killing for antigen-negative tumor cells.

    Article Title: Phocaeicola vulgatus induces immunotherapy resistance in hepatocellular carcinoma via reducing indoleacetic acid production.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Mouse IL-2 Recombinant Protein PeproTech Cat#212-12 Recombinant Human IL-2 R&D systems Cat202-IL-010 Human T cell TransAct Miltenyi Cat#130-111-160 Neomycin Sigma-Aldrich Cat#N5285-25G Metronidazole Sigma-Aldrich Cat#M1547-25G Vancomycin Sigma-Aldrich Cat#V8138-1G Ampicillin Sigma-Aldrich Cat#A1593-25G Indole-3-Acetic Acid Sodium Salt Sigma-Aldrich Cat#I5148 Dulbecco’s Modified Eagle Medium Gibco Cat#C11995500BT RPMI 1640 Medium Gibco Cat#C11875500BT PBS Gibco Cat#C20012500BT TRYPSIN 0.25% EDTA Gibco Cat#25200072 Fetal Bovine Serum Thermo Fisher Cat#10270-106 Penicillin-Streptomycin Gibco Cat#15140-122 TexMAC GMP Medium Miltenyi Cat#170-076-309 2-Mercaptoethanol Gibico Cat#21985023 D-Luciferin PerkinElmer Cat#122799 DNase I Roche Cat#10104159001 Liberase TM Research Grade Roche Cat#05401119001 Percoll GE Healthcare Cat#17-0891-01 RBC Lysis Buffer BioLegend Cat#420301 Cell Staining Buffer BioLegend Cat#420201 Leukocyte Activation Cocktail BD Biosciences Cat#550583 Protein Transport Inhibitor BD Biosciences Cat#555029 Triton X-100 Thermo Fisher Cat#85111 DAPI Thermo Fisher Cat#S36938 BSA Sigma‒Aldrich Cat#A9418 L-Amino Acid Rodent Diet without Added Tryptohan SYSE Bio-Tec. .. Co., Ltd. Cat#A22071501 L-Amino Acid Standard Rodent Diet SYSE Bio-Tec.

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Human IL-3 PEPROTECH Cat# 200-03 Human IL-6 PEPROTECH Cat# 200-06 Human SCF PEPROTECH Cat# 300-07 Human TPO PEPROTECH Cat# 300-18 Human IL-2 R&D Systems Cat# BT-002 Mouse IL-2 R&D Systems Cat# 402-ML APC-conjugated H-2Kb OVA Tetramer-SIINFEKL MBL Cat# TS-5001-2C Protease inhibitor cocktail Selleck Cat# B14001 Phosphatase inhibitor cocktail Selleck Cat# B15001 Sulfosuccinimidyl oleate MedChemExpress Cat# HY-112847 SC75741 MedChemExpress Cat# HY-10496 Fluvastatin MedChemExpress Cat# HY-14664 C75 MedChemExpress Cat# HY-12364 Bezafibrate MedChemExpress Cat# HY-B0637 Etomoxir MedChemExpress Cat# HY-50202 ABT-737 MedChemExpress Cat# HY-50907 Cytarabine MedChemExpress Cat# HY-13605 Coumermycin A1 MedChemExpress Cat# HY-N7452 Novobiocin MedChemExpress Cat# HY-B0425A TAK-242 MedChemExpress Cat# HY-11109 PP1 MedChemExpress Cat# HY-13804 Decitabine MedChemExpress Cat# HY-A0004 a-Tocopherol MedChemExpress Cat# HY-N0683 Busulfan MedChemExpress Cat# HY-B0245 Nor-NOHA ApexBio Technology Cat# C5407 OxLDL Solarbio Cat# IL2150 LDL Solarbio Cat# IL2110 HDL Solarbio Cat# IL2130 Palmitate Cayman Chemical Cat# 10006627 Oleic acid Cayman Chemical Cat# 90260 Arachidonic Acid Cayman Chemical Cat# 90010 CFSE Biolegend Cat# 423801 SIINFEKL peptide Nanjing Peptide Biotech N/A alkynyl palmitate click chemistry tools Cat# 1165 Critical commercial assays StemSpan SFEM II STEMCELL Technologies Cat# 09605_C EasySep Human Cord Blood CD34 Positive Selection Kit III STEMCELL Technologies Cat# 17897 (Continued on next page) e2 Cell Reports Medicine 5, 101592, June 18, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein Enrichment Kit for capture of alkynemodified proteins click chemistry tools Cat# 1441 Fatty Acid Uptake Assay Kit Abnova Cat# KA4084 Oxidized LDL Uptake Assay Kit Cayman Chemical Cat# 601180 Nuclear and Cytoplasmic Protein Extraction Kit Sangon Biotech Cat# C510001 Pan T cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-535 Pan T cell Isolation Kit II, mouse Miltenyi Biotec Cat# 130-095-130 Mouse CD8+ T cell Isolation Kit STEMCELL Technologies Cat# 19853 T cell Activation/Expansion Kit, mouse Miltenyi Biotec Cat# 130-093-627 T cell Activation/Expansion Kit, human Miltenyi Biotec Cat# 130-091-441 Deposited data ChIP-seq data of p65 Lin et al.45 GSE103477 RNA-seq data of CD36KO vs. CD36NC MV4-11 cells This paper GSE209783 RNA-seq data of decitabine-treated vs. untreated THP-1 cells This paper GSE200510 RNA-seq data of decitabine-treated vs. untreated MV4-11 cells This paper GSE225384 RNA-seq data of AML patients Li et al.34 GSE37642 Experimental models: Cell lines MV4-11 ATCC Cat# CRL-9591 HEK-293T ATCC Cat# CRL-3216 MOLM-13 DSMZ Cat# ACC 544 OCIAML2 DSMZ Cat# ACC 99 OCIAML3 DSMZ Cat# ACC 582 NOMO1 DSMZ Cat# ACC 542 SET2 DSMZ Cat# ACC 608 U937 ATCC Cat# CRL-1593.2 C1498 ATCC Cat# TIB-49 THP-1 ATCC Cat# TIB-202 NB4 DSMZ Cat# ACC 207 K-562 ATCC Cat# CCL-243 Experimental models: Organisms/strains NOD.Cg-PrkdcscidIl2rgtm1Sug/JicCrl (NOG) mice Vital River Cat# 408 C57BL/6JNifdc mice Vital River Cat# 219 NOD.Cg-PrkdcscidIl2rgtm1SugB2mem1TacH2-Ab1tm1Doi/ JicCrl (NOG-dKO) mice Vital River Cat# 411 Oligonucleotides shRNA sequences This paper Table S4 sgRNA sequences This paper Table S4 primers used for RT-PCR This paper Table S4 primers used for NGS-BSP This paper Table S4 Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 LentiGuide Puro-P2A-EGFP Addgene Cat# 137729 lentiCas9-Blast Addgene Cat# 52962 pLKO.5 Sigma-Aldrich Cat# SHC201 pLV-EF1a-IRES-Neo Addgene Cat# 85139 (Continued on next page) Cell Reports Medicine 5, 101592, June 18, 2024 e3

    Protease Inhibitor:

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies phospho-NF-kB p65 (Ser536) Cell Signaling Technology Cat# 3033S; RRID: AB_331284 NF-kB p65 Cell Signaling Technology Cat# 8242S; RRID: AB_10859369 b-Actin Cell Signaling Technology Cat# 3700S; RRID: AB_2242334 MYD88 Cell Signaling Technology Cat# 4283S; RRID: AB_10547882 LYN Cell Signaling Technology Cat# 2796S; RRID: AB_2138391 Lamin-B1 Santa Cruz Cat# sc-374015; RRID: AB_10947408 MYD88 Santa Cruz Cat# sc-74532; RRID: AB_1126429 TLR4 Santa Cruz Cat# sc-293072; AB_10611320 LYN Santa Cruz Cat# sc-7274; AB_627897 Normal mouse IgG Santa Cruz Cat# sc-2025; AB_737182 CD36 Abcam Cat# ab133625; RRID: AB_2716564 ZDHHC6 Abcam Cat# ab121423; RRID: AB_11129943 CD3 Abcam Cat# ab135372; RRID: AB_2884903 TLR4 CUSABIO Technology Cat# CSB-PA001434 InVivoPlus anti-mouse NK1.1 BioXcell Cat# BE0036; RRID: AB_1107737 InVivoMab anti-mouse CD8b BioXcell Cat# BE0223; RRID: AB_2687706 anti-mouse CD4 BioXcell Cat# BP0003-1; RRID: AB_1107636 InVivoPlus anti-mouse PD-L1 BioXcell Cat# BP0101; RRID: AB_10949073 InVivoPlus mouse IgG2a BioXcell Cat# BE0085; RRID: AB_1107771 InVivoPlus rat IgG1 BioXcell Cat# BE0088; RRID: AB_1107775 InVivoPlus rat IgG2b BioXcell Cat# BP0090; RRID: AB_1107780 Phospho-NFkB p65 (Ser529) Antibody, PE Invitrogen Cat# MA5-37165; RRID: AB_2897099 Arginase 1 Antibody, APC Invitrogen Cat# 17-3697-80; RRID: AB_2734834 Anti-human PD-L1-PE-Cy7 Invitrogen Cat# 25-5983-41; RRID: AB_1907369 Anti-mouse PD-1-BV785 BioLegend Cat# 135225; RRID: AB_2563680 Anti-mouse LAG-3-BV785 BioLegend Cat# 125219; RRID: AB_2566571 Anti-human PD-1-PE-Cy7 BioLegend Cat# 329917; RRID: AB_2159325 Anti-human CD8-FITC BioLegend Cat# 344704; RRID: AB_1877178 Anti-human CD14-BV421 BioLegend Cat# 301830; RRID: AB_10959324 Anti-mouse TIM3-PE eBioscience Cat# 12-5870-82; RRID: AB_465974 OVA257-264 (SIINFEKL) peptide bound to H-2K b Antibody, APC eBioscience Cat# 17-5743-80; RRID: AB_1311288 Anti-mouse CD36-APC eBioscience Cat# 17-0362-82; RRID: AB_2734967 Anti-mouse PD-L1-PE-Cy7 eBioscience Cat# 25-5982-82; RRID: AB_2573509 Anti-human TLR4-APC eBioscience Cat# 17-9917-41; RRID: AB_2016622 Anti-mouse CD107a-BV421 BD Biosciences Cat# 564347; RRID: AB_2738760 Anti-human CD33-BV421 BD Biosciences Cat# 562854; RRID: AB_2737405 Anti-human CD36-APC BD Biosciences Cat# 561822; RRID: AB_398480 Anti-human CD36-FITC BD Biosciences Cat# 555454; RRID: AB_2291112 Anti-human CD34-FITC BD Biosciences Cat# 555821; RRID: AB_396150 Anti-human CD34-APC BD Biosciences Cat# 561209; RRID: AB_10683161 Anti-human CD45-APC-Cy7 BD Biosciences Cat# 557833; RRID: AB_396891 Anti-human CD56-PE-Cy5 BD Biosciences Cat# 555517; RRID: AB_395907 (Continued on next page) Cell Reports Medicine 5, 101592, June 18, 2024 e1 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Human IL-3 PEPROTECH Cat# 200-03 Human IL-6 PEPROTECH Cat# 200-06 Human SCF PEPROTECH Cat# 300-07 Human TPO PEPROTECH Cat# 300-18 Human IL-2 R&D Systems Cat# BT-002 Mouse IL-2 R&D Systems Cat# 402-ML APC-conjugated H-2Kb OVA Tetramer-SIINFEKL MBL Cat# TS-5001-2C Protease inhibitor cocktail Selleck Cat# B14001 Phosphatase inhibitor cocktail Selleck Cat# B15001 Sulfosuccinimidyl oleate MedChemExpress Cat# HY-112847 SC75741 MedChemExpress Cat# HY-10496 Fluvastatin MedChemExpress Cat# HY-14664 C75 MedChemExpress Cat# HY-12364 Bezafibrate MedChemExpress Cat# HY-B0637 Etomoxir MedChemExpress Cat# HY-50202 ABT-737 MedChemExpress Cat# HY-50907 Cytarabine MedChemExpress Cat# HY-13605 Coumermycin A1 MedChemExpress Cat# HY-N7452 Novobiocin MedChemExpress Cat# HY-B0425A TAK-242 MedChemExpress Cat# HY-11109 PP1 MedChemExpress Cat# HY-13804 Decitabine MedChemExpress Cat# HY-A0004 a-Tocopherol MedChemExpress Cat# HY-N0683 Busulfan MedChemExpress Cat# HY-B0245 Nor-NOHA ApexBio Technology Cat# C5407 OxLDL Solarbio Cat# IL2150 LDL Solarbio Cat# IL2110 HDL Solarbio Cat# IL2130 Palmitate Cayman Chemical Cat# 10006627 Oleic acid Cayman Chemical Cat# 90260 Arachidonic Acid Cayman Chemical Cat# 90010 CFSE Biolegend Cat# 423801 SIINFEKL peptide Nanjing Peptide Biotech N/A alkynyl palmitate click chemistry tools Cat# 1165 Critical commercial assays StemSpan SFEM II STEMCELL Technologies Cat# 09605_C EasySep Human Cord Blood CD34 Positive Selection Kit III STEMCELL Technologies Cat# 17897 (Continued on next page) e2 Cell Reports Medicine 5, 101592, June 18, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein Enrichment Kit for capture of alkynemodified proteins click chemistry tools Cat# 1441 Fatty Acid Uptake Assay Kit Abnova Cat# KA4084 Oxidized LDL Uptake Assay Kit Cayman Chemical Cat# 601180 Nuclear and Cytoplasmic Protein Extraction Kit Sangon Biotech Cat# C510001 Pan T cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-535 Pan T cell Isolation Kit II, mouse Miltenyi Biotec Cat# 130-095-130 Mouse CD8+ T cell Isolation Kit STEMCELL Technologies Cat# 19853 T cell Activation/Expansion Kit, mouse Miltenyi Biotec Cat# 130-093-627 T cell Activation/Expansion Kit, human Miltenyi Biotec Cat# 130-091-441 Deposited data ChIP-seq data of p65 Lin et al.45 GSE103477 RNA-seq data of CD36KO vs. CD36NC MV4-11 cells This paper GSE209783 RNA-seq data of decitabine-treated vs. untreated THP-1 cells This paper GSE200510 RNA-seq data of decitabine-treated vs. untreated MV4-11 cells This paper GSE225384 RNA-seq data of AML patients Li et al.34 GSE37642 Experimental models: Cell lines MV4-11 ATCC Cat# CRL-9591 HEK-293T ATCC Cat# CRL-3216 MOLM-13 DSMZ Cat# ACC 544 OCIAML2 DSMZ Cat# ACC 99 OCIAML3 DSMZ Cat# ACC 582 NOMO1 DSMZ Cat# ACC 542 SET2 DSMZ Cat# ACC 608 U937 ATCC Cat# CRL-1593.2 C1498 ATCC Cat# TIB-49 THP-1 ATCC Cat# TIB-202 NB4 DSMZ Cat# ACC 207 K-562 ATCC Cat# CCL-243 Experimental models: Organisms/strains NOD.Cg-PrkdcscidIl2rgtm1Sug/JicCrl (NOG) mice Vital River Cat# 408 C57BL/6JNifdc mice Vital River Cat# 219 NOD.Cg-PrkdcscidIl2rgtm1SugB2mem1TacH2-Ab1tm1Doi/ JicCrl (NOG-dKO) mice Vital River Cat# 411 Oligonucleotides shRNA sequences This paper Table S4 sgRNA sequences This paper Table S4 primers used for RT-PCR This paper Table S4 primers used for NGS-BSP This paper Table S4 Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 LentiGuide Puro-P2A-EGFP Addgene Cat# 137729 lentiCas9-Blast Addgene Cat# 52962 pLKO.5 Sigma-Aldrich Cat# SHC201 pLV-EF1a-IRES-Neo Addgene Cat# 85139 (Continued on next page) Cell Reports Medicine 5, 101592, June 18, 2024 e3 Article ll OPEN ACCESS

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Human IL-3 PEPROTECH Cat# 200-03 Human IL-6 PEPROTECH Cat# 200-06 Human SCF PEPROTECH Cat# 300-07 Human TPO PEPROTECH Cat# 300-18 Human IL-2 R&D Systems Cat# BT-002 Mouse IL-2 R&D Systems Cat# 402-ML APC-conjugated H-2Kb OVA Tetramer-SIINFEKL MBL Cat# TS-5001-2C Protease inhibitor cocktail Selleck Cat# B14001 Phosphatase inhibitor cocktail Selleck Cat# B15001 Sulfosuccinimidyl oleate MedChemExpress Cat# HY-112847 SC75741 MedChemExpress Cat# HY-10496 Fluvastatin MedChemExpress Cat# HY-14664 C75 MedChemExpress Cat# HY-12364 Bezafibrate MedChemExpress Cat# HY-B0637 Etomoxir MedChemExpress Cat# HY-50202 ABT-737 MedChemExpress Cat# HY-50907 Cytarabine MedChemExpress Cat# HY-13605 Coumermycin A1 MedChemExpress Cat# HY-N7452 Novobiocin MedChemExpress Cat# HY-B0425A TAK-242 MedChemExpress Cat# HY-11109 PP1 MedChemExpress Cat# HY-13804 Decitabine MedChemExpress Cat# HY-A0004 a-Tocopherol MedChemExpress Cat# HY-N0683 Busulfan MedChemExpress Cat# HY-B0245 Nor-NOHA ApexBio Technology Cat# C5407 OxLDL Solarbio Cat# IL2150 LDL Solarbio Cat# IL2110 HDL Solarbio Cat# IL2130 Palmitate Cayman Chemical Cat# 10006627 Oleic acid Cayman Chemical Cat# 90260 Arachidonic Acid Cayman Chemical Cat# 90010 CFSE Biolegend Cat# 423801 SIINFEKL peptide Nanjing Peptide Biotech N/A alkynyl palmitate click chemistry tools Cat# 1165 Critical commercial assays StemSpan SFEM II STEMCELL Technologies Cat# 09605_C EasySep Human Cord Blood CD34 Positive Selection Kit III STEMCELL Technologies Cat# 17897 (Continued on next page) e2 Cell Reports Medicine 5, 101592, June 18, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein Enrichment Kit for capture of alkynemodified proteins click chemistry tools Cat# 1441 Fatty Acid Uptake Assay Kit Abnova Cat# KA4084 Oxidized LDL Uptake Assay Kit Cayman Chemical Cat# 601180 Nuclear and Cytoplasmic Protein Extraction Kit Sangon Biotech Cat# C510001 Pan T cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-535 Pan T cell Isolation Kit II, mouse Miltenyi Biotec Cat# 130-095-130 Mouse CD8+ T cell Isolation Kit STEMCELL Technologies Cat# 19853 T cell Activation/Expansion Kit, mouse Miltenyi Biotec Cat# 130-093-627 T cell Activation/Expansion Kit, human Miltenyi Biotec Cat# 130-091-441 Deposited data ChIP-seq data of p65 Lin et al.45 GSE103477 RNA-seq data of CD36KO vs. CD36NC MV4-11 cells This paper GSE209783 RNA-seq data of decitabine-treated vs. untreated THP-1 cells This paper GSE200510 RNA-seq data of decitabine-treated vs. untreated MV4-11 cells This paper GSE225384 RNA-seq data of AML patients Li et al.34 GSE37642 Experimental models: Cell lines MV4-11 ATCC Cat# CRL-9591 HEK-293T ATCC Cat# CRL-3216 MOLM-13 DSMZ Cat# ACC 544 OCIAML2 DSMZ Cat# ACC 99 OCIAML3 DSMZ Cat# ACC 582 NOMO1 DSMZ Cat# ACC 542 SET2 DSMZ Cat# ACC 608 U937 ATCC Cat# CRL-1593.2 C1498 ATCC Cat# TIB-49 THP-1 ATCC Cat# TIB-202 NB4 DSMZ Cat# ACC 207 K-562 ATCC Cat# CCL-243 Experimental models: Organisms/strains NOD.Cg-PrkdcscidIl2rgtm1Sug/JicCrl (NOG) mice Vital River Cat# 408 C57BL/6JNifdc mice Vital River Cat# 219 NOD.Cg-PrkdcscidIl2rgtm1SugB2mem1TacH2-Ab1tm1Doi/ JicCrl (NOG-dKO) mice Vital River Cat# 411 Oligonucleotides shRNA sequences This paper Table S4 sgRNA sequences This paper Table S4 primers used for RT-PCR This paper Table S4 primers used for NGS-BSP This paper Table S4 Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 LentiGuide Puro-P2A-EGFP Addgene Cat# 137729 lentiCas9-Blast Addgene Cat# 52962 pLKO.5 Sigma-Aldrich Cat# SHC201 pLV-EF1a-IRES-Neo Addgene Cat# 85139 (Continued on next page) Cell Reports Medicine 5, 101592, June 18, 2024 e3

    Selection:

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies phospho-NF-kB p65 (Ser536) Cell Signaling Technology Cat# 3033S; RRID: AB_331284 NF-kB p65 Cell Signaling Technology Cat# 8242S; RRID: AB_10859369 b-Actin Cell Signaling Technology Cat# 3700S; RRID: AB_2242334 MYD88 Cell Signaling Technology Cat# 4283S; RRID: AB_10547882 LYN Cell Signaling Technology Cat# 2796S; RRID: AB_2138391 Lamin-B1 Santa Cruz Cat# sc-374015; RRID: AB_10947408 MYD88 Santa Cruz Cat# sc-74532; RRID: AB_1126429 TLR4 Santa Cruz Cat# sc-293072; AB_10611320 LYN Santa Cruz Cat# sc-7274; AB_627897 Normal mouse IgG Santa Cruz Cat# sc-2025; AB_737182 CD36 Abcam Cat# ab133625; RRID: AB_2716564 ZDHHC6 Abcam Cat# ab121423; RRID: AB_11129943 CD3 Abcam Cat# ab135372; RRID: AB_2884903 TLR4 CUSABIO Technology Cat# CSB-PA001434 InVivoPlus anti-mouse NK1.1 BioXcell Cat# BE0036; RRID: AB_1107737 InVivoMab anti-mouse CD8b BioXcell Cat# BE0223; RRID: AB_2687706 anti-mouse CD4 BioXcell Cat# BP0003-1; RRID: AB_1107636 InVivoPlus anti-mouse PD-L1 BioXcell Cat# BP0101; RRID: AB_10949073 InVivoPlus mouse IgG2a BioXcell Cat# BE0085; RRID: AB_1107771 InVivoPlus rat IgG1 BioXcell Cat# BE0088; RRID: AB_1107775 InVivoPlus rat IgG2b BioXcell Cat# BP0090; RRID: AB_1107780 Phospho-NFkB p65 (Ser529) Antibody, PE Invitrogen Cat# MA5-37165; RRID: AB_2897099 Arginase 1 Antibody, APC Invitrogen Cat# 17-3697-80; RRID: AB_2734834 Anti-human PD-L1-PE-Cy7 Invitrogen Cat# 25-5983-41; RRID: AB_1907369 Anti-mouse PD-1-BV785 BioLegend Cat# 135225; RRID: AB_2563680 Anti-mouse LAG-3-BV785 BioLegend Cat# 125219; RRID: AB_2566571 Anti-human PD-1-PE-Cy7 BioLegend Cat# 329917; RRID: AB_2159325 Anti-human CD8-FITC BioLegend Cat# 344704; RRID: AB_1877178 Anti-human CD14-BV421 BioLegend Cat# 301830; RRID: AB_10959324 Anti-mouse TIM3-PE eBioscience Cat# 12-5870-82; RRID: AB_465974 OVA257-264 (SIINFEKL) peptide bound to H-2K b Antibody, APC eBioscience Cat# 17-5743-80; RRID: AB_1311288 Anti-mouse CD36-APC eBioscience Cat# 17-0362-82; RRID: AB_2734967 Anti-mouse PD-L1-PE-Cy7 eBioscience Cat# 25-5982-82; RRID: AB_2573509 Anti-human TLR4-APC eBioscience Cat# 17-9917-41; RRID: AB_2016622 Anti-mouse CD107a-BV421 BD Biosciences Cat# 564347; RRID: AB_2738760 Anti-human CD33-BV421 BD Biosciences Cat# 562854; RRID: AB_2737405 Anti-human CD36-APC BD Biosciences Cat# 561822; RRID: AB_398480 Anti-human CD36-FITC BD Biosciences Cat# 555454; RRID: AB_2291112 Anti-human CD34-FITC BD Biosciences Cat# 555821; RRID: AB_396150 Anti-human CD34-APC BD Biosciences Cat# 561209; RRID: AB_10683161 Anti-human CD45-APC-Cy7 BD Biosciences Cat# 557833; RRID: AB_396891 Anti-human CD56-PE-Cy5 BD Biosciences Cat# 555517; RRID: AB_395907 (Continued on next page) Cell Reports Medicine 5, 101592, June 18, 2024 e1 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Human IL-3 PEPROTECH Cat# 200-03 Human IL-6 PEPROTECH Cat# 200-06 Human SCF PEPROTECH Cat# 300-07 Human TPO PEPROTECH Cat# 300-18 Human IL-2 R&D Systems Cat# BT-002 Mouse IL-2 R&D Systems Cat# 402-ML APC-conjugated H-2Kb OVA Tetramer-SIINFEKL MBL Cat# TS-5001-2C Protease inhibitor cocktail Selleck Cat# B14001 Phosphatase inhibitor cocktail Selleck Cat# B15001 Sulfosuccinimidyl oleate MedChemExpress Cat# HY-112847 SC75741 MedChemExpress Cat# HY-10496 Fluvastatin MedChemExpress Cat# HY-14664 C75 MedChemExpress Cat# HY-12364 Bezafibrate MedChemExpress Cat# HY-B0637 Etomoxir MedChemExpress Cat# HY-50202 ABT-737 MedChemExpress Cat# HY-50907 Cytarabine MedChemExpress Cat# HY-13605 Coumermycin A1 MedChemExpress Cat# HY-N7452 Novobiocin MedChemExpress Cat# HY-B0425A TAK-242 MedChemExpress Cat# HY-11109 PP1 MedChemExpress Cat# HY-13804 Decitabine MedChemExpress Cat# HY-A0004 a-Tocopherol MedChemExpress Cat# HY-N0683 Busulfan MedChemExpress Cat# HY-B0245 Nor-NOHA ApexBio Technology Cat# C5407 OxLDL Solarbio Cat# IL2150 LDL Solarbio Cat# IL2110 HDL Solarbio Cat# IL2130 Palmitate Cayman Chemical Cat# 10006627 Oleic acid Cayman Chemical Cat# 90260 Arachidonic Acid Cayman Chemical Cat# 90010 CFSE Biolegend Cat# 423801 SIINFEKL peptide Nanjing Peptide Biotech N/A alkynyl palmitate click chemistry tools Cat# 1165 Critical commercial assays StemSpan SFEM II STEMCELL Technologies Cat# 09605_C EasySep Human Cord Blood CD34 Positive Selection Kit III STEMCELL Technologies Cat# 17897 (Continued on next page) e2 Cell Reports Medicine 5, 101592, June 18, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein Enrichment Kit for capture of alkynemodified proteins click chemistry tools Cat# 1441 Fatty Acid Uptake Assay Kit Abnova Cat# KA4084 Oxidized LDL Uptake Assay Kit Cayman Chemical Cat# 601180 Nuclear and Cytoplasmic Protein Extraction Kit Sangon Biotech Cat# C510001 Pan T cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-535 Pan T cell Isolation Kit II, mouse Miltenyi Biotec Cat# 130-095-130 Mouse CD8+ T cell Isolation Kit STEMCELL Technologies Cat# 19853 T cell Activation/Expansion Kit, mouse Miltenyi Biotec Cat# 130-093-627 T cell Activation/Expansion Kit, human Miltenyi Biotec Cat# 130-091-441 Deposited data ChIP-seq data of p65 Lin et al.45 GSE103477 RNA-seq data of CD36KO vs. CD36NC MV4-11 cells This paper GSE209783 RNA-seq data of decitabine-treated vs. untreated THP-1 cells This paper GSE200510 RNA-seq data of decitabine-treated vs. untreated MV4-11 cells This paper GSE225384 RNA-seq data of AML patients Li et al.34 GSE37642 Experimental models: Cell lines MV4-11 ATCC Cat# CRL-9591 HEK-293T ATCC Cat# CRL-3216 MOLM-13 DSMZ Cat# ACC 544 OCIAML2 DSMZ Cat# ACC 99 OCIAML3 DSMZ Cat# ACC 582 NOMO1 DSMZ Cat# ACC 542 SET2 DSMZ Cat# ACC 608 U937 ATCC Cat# CRL-1593.2 C1498 ATCC Cat# TIB-49 THP-1 ATCC Cat# TIB-202 NB4 DSMZ Cat# ACC 207 K-562 ATCC Cat# CCL-243 Experimental models: Organisms/strains NOD.Cg-PrkdcscidIl2rgtm1Sug/JicCrl (NOG) mice Vital River Cat# 408 C57BL/6JNifdc mice Vital River Cat# 219 NOD.Cg-PrkdcscidIl2rgtm1SugB2mem1TacH2-Ab1tm1Doi/ JicCrl (NOG-dKO) mice Vital River Cat# 411 Oligonucleotides shRNA sequences This paper Table S4 sgRNA sequences This paper Table S4 primers used for RT-PCR This paper Table S4 primers used for NGS-BSP This paper Table S4 Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 LentiGuide Puro-P2A-EGFP Addgene Cat# 137729 lentiCas9-Blast Addgene Cat# 52962 pLKO.5 Sigma-Aldrich Cat# SHC201 pLV-EF1a-IRES-Neo Addgene Cat# 85139 (Continued on next page) Cell Reports Medicine 5, 101592, June 18, 2024 e3 Article ll OPEN ACCESS

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Human IL-3 PEPROTECH Cat# 200-03 Human IL-6 PEPROTECH Cat# 200-06 Human SCF PEPROTECH Cat# 300-07 Human TPO PEPROTECH Cat# 300-18 Human IL-2 R&D Systems Cat# BT-002 Mouse IL-2 R&D Systems Cat# 402-ML APC-conjugated H-2Kb OVA Tetramer-SIINFEKL MBL Cat# TS-5001-2C Protease inhibitor cocktail Selleck Cat# B14001 Phosphatase inhibitor cocktail Selleck Cat# B15001 Sulfosuccinimidyl oleate MedChemExpress Cat# HY-112847 SC75741 MedChemExpress Cat# HY-10496 Fluvastatin MedChemExpress Cat# HY-14664 C75 MedChemExpress Cat# HY-12364 Bezafibrate MedChemExpress Cat# HY-B0637 Etomoxir MedChemExpress Cat# HY-50202 ABT-737 MedChemExpress Cat# HY-50907 Cytarabine MedChemExpress Cat# HY-13605 Coumermycin A1 MedChemExpress Cat# HY-N7452 Novobiocin MedChemExpress Cat# HY-B0425A TAK-242 MedChemExpress Cat# HY-11109 PP1 MedChemExpress Cat# HY-13804 Decitabine MedChemExpress Cat# HY-A0004 a-Tocopherol MedChemExpress Cat# HY-N0683 Busulfan MedChemExpress Cat# HY-B0245 Nor-NOHA ApexBio Technology Cat# C5407 OxLDL Solarbio Cat# IL2150 LDL Solarbio Cat# IL2110 HDL Solarbio Cat# IL2130 Palmitate Cayman Chemical Cat# 10006627 Oleic acid Cayman Chemical Cat# 90260 Arachidonic Acid Cayman Chemical Cat# 90010 CFSE Biolegend Cat# 423801 SIINFEKL peptide Nanjing Peptide Biotech N/A alkynyl palmitate click chemistry tools Cat# 1165 Critical commercial assays StemSpan SFEM II STEMCELL Technologies Cat# 09605_C EasySep Human Cord Blood CD34 Positive Selection Kit III STEMCELL Technologies Cat# 17897 (Continued on next page) e2 Cell Reports Medicine 5, 101592, June 18, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein Enrichment Kit for capture of alkynemodified proteins click chemistry tools Cat# 1441 Fatty Acid Uptake Assay Kit Abnova Cat# KA4084 Oxidized LDL Uptake Assay Kit Cayman Chemical Cat# 601180 Nuclear and Cytoplasmic Protein Extraction Kit Sangon Biotech Cat# C510001 Pan T cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-535 Pan T cell Isolation Kit II, mouse Miltenyi Biotec Cat# 130-095-130 Mouse CD8+ T cell Isolation Kit STEMCELL Technologies Cat# 19853 T cell Activation/Expansion Kit, mouse Miltenyi Biotec Cat# 130-093-627 T cell Activation/Expansion Kit, human Miltenyi Biotec Cat# 130-091-441 Deposited data ChIP-seq data of p65 Lin et al.45 GSE103477 RNA-seq data of CD36KO vs. CD36NC MV4-11 cells This paper GSE209783 RNA-seq data of decitabine-treated vs. untreated THP-1 cells This paper GSE200510 RNA-seq data of decitabine-treated vs. untreated MV4-11 cells This paper GSE225384 RNA-seq data of AML patients Li et al.34 GSE37642 Experimental models: Cell lines MV4-11 ATCC Cat# CRL-9591 HEK-293T ATCC Cat# CRL-3216 MOLM-13 DSMZ Cat# ACC 544 OCIAML2 DSMZ Cat# ACC 99 OCIAML3 DSMZ Cat# ACC 582 NOMO1 DSMZ Cat# ACC 542 SET2 DSMZ Cat# ACC 608 U937 ATCC Cat# CRL-1593.2 C1498 ATCC Cat# TIB-49 THP-1 ATCC Cat# TIB-202 NB4 DSMZ Cat# ACC 207 K-562 ATCC Cat# CCL-243 Experimental models: Organisms/strains NOD.Cg-PrkdcscidIl2rgtm1Sug/JicCrl (NOG) mice Vital River Cat# 408 C57BL/6JNifdc mice Vital River Cat# 219 NOD.Cg-PrkdcscidIl2rgtm1SugB2mem1TacH2-Ab1tm1Doi/ JicCrl (NOG-dKO) mice Vital River Cat# 411 Oligonucleotides shRNA sequences This paper Table S4 sgRNA sequences This paper Table S4 primers used for RT-PCR This paper Table S4 primers used for NGS-BSP This paper Table S4 Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 LentiGuide Puro-P2A-EGFP Addgene Cat# 137729 lentiCas9-Blast Addgene Cat# 52962 pLKO.5 Sigma-Aldrich Cat# SHC201 pLV-EF1a-IRES-Neo Addgene Cat# 85139 (Continued on next page) Cell Reports Medicine 5, 101592, June 18, 2024 e3

    other:


    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Protein Enrichment Kit for capture of alkynemodified proteins click chemistry tools Cat# 1441 Fatty Acid Uptake Assay Kit Abnova Cat# KA4084 Oxidized LDL Uptake Assay Kit Cayman Chemical Cat# 601180 Nuclear and Cytoplasmic Protein Extraction Kit Sangon Biotech Cat# C510001 Pan T cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-535 Pan T cell Isolation Kit II, mouse Miltenyi Biotec Cat# 130-095-130 Mouse CD8+ T cell Isolation Kit STEMCELL Technologies Cat# 19853 T cell Activation/Expansion Kit, mouse Miltenyi Biotec Cat# 130-093-627 T cell Activation/Expansion Kit, human Miltenyi Biotec Cat# 130-091-441 Deposited data ChIP-seq data of p65 Lin et al.45 GSE103477 RNA-seq data of CD36KO vs. CD36NC MV4-11 cells This paper GSE209783 RNA-seq data of decitabine-treated vs. untreated THP-1 cells This paper GSE200510 RNA-seq data of decitabine-treated vs. untreated MV4-11 cells This paper GSE225384 RNA-seq data of AML patients Li et al.34 GSE37642 Experimental models: Cell lines MV4-11 ATCC Cat# CRL-9591 HEK-293T ATCC Cat# CRL-3216 MOLM-13 DSMZ Cat# ACC 544 OCIAML2 DSMZ Cat# ACC 99 OCIAML3 DSMZ Cat# ACC 582 NOMO1 DSMZ Cat# ACC 542 SET2 DSMZ Cat# ACC 608 U937 ATCC Cat# CRL-1593.2 C1498 ATCC Cat# TIB-49 THP-1 ATCC Cat# TIB-202 NB4 DSMZ Cat# ACC 207 K-562 ATCC Cat# CCL-243 Experimental models: Organisms/strains NOD.Cg-PrkdcscidIl2rgtm1Sug/JicCrl (NOG) mice Vital River Cat# 408 C57BL/6JNifdc mice Vital River Cat# 219 NOD.Cg-PrkdcscidIl2rgtm1SugB2mem1TacH2-Ab1tm1Doi/ JicCrl (NOG-dKO) mice Vital River Cat# 411 Oligonucleotides shRNA sequences This paper Table S4 sgRNA sequences This paper Table S4 primers used for RT-PCR This paper Table S4 primers used for NGS-BSP This paper Table S4 Recombinant DNA pMD2.G Addgene Cat# 12259 psPAX2 Addgene Cat# 12260 LentiGuide Puro-P2A-EGFP Addgene Cat# 137729 lentiCas9-Blast Addgene Cat# 52962 pLKO.5 Sigma-Aldrich Cat# SHC201 pLV-EF1a-IRES-Neo Addgene Cat# 85139 (Continued on next page) Cell Reports Medicine 5, 101592, June 18, 2024 e3

    Virus:

    Article Title: GATA1-mediated Notch signaling augment antitumor immunity of CD11b + CD27 - natural killer cells maturation via BCL9/β-catenin signal.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse-CD45-PerCP/Cy5.5 Invitrogen Cat#45-0451-82; RRID:AB_1107002 Anti-mouse-CD3e-eFluor 450 Invitrogen Cat#48-0031-80; RRID:AB_10733280 Anti-human/mouse-Granzyme B-Alexa Fluor 700 Biolegend Cat#372222; RRID:AB_2728389 Anti-mouse-Granzyme B-eFluor 450 eBioscience Cat#48-8898-82; RRID:AB_11149362 Anti-mouse-CD335 (NKp46)-Brilliant Violet 605 Biolegend Cat#137619; RRID:AB_2562452 Anti-mouse-CD335 (NKp46)-FITC eBioscience Cat#11-3351-82; RRID:AB_1210843 Anti-mouse-CD314 (NKG2D)-Alexa Fluor 488 eBioscience Cat#53-5882-82; RRID:AB_2811867 Anti-mouse-NK1.1-APC BioLegend Cat#108709; RRID:AB_313396 Anti-mouse-CD27-PE Biolegend Cat#124210; RRID:AB_1236459 Anti-mouse-CD11b-PE/Cy7 Biolegend Cat#10216 Anti-human-CD16-PE BioLegend Cat#360704; RRID:AB_2562749 Anti-human-CD56-APC BioLegend Cat#362504; RRID:AB_2563913 Anti-mouse-Ki-67-PE eBioscience Cat#12-5698-80; RRID:AB_11149672 Anti-human -CD3e-FITC MULTISCIENCES Cat#AH00301-100; RRID:AB_2890114 Anti-mouse -CD3e-FITC MULTISCIENCES Cat#AM003E0201-250 NOTCH1 Polyclonal antibody Proteintech Cat#20687-1-AP; RRID:AB_10700012 β-Actin Proteintech Cat#66009-1-Ig; RRID:AB_2687938 Bacterial and virus strains HBAAV2/9-ZsGreen Hanbio Cat#75010302 HBAAV2/9-Klrb1c-m-cre-3xflag-ZsGreen Hanbio Cat#81071807 Cignal Reporter Assay Kits QIAGEN Cat# CCS018L Chemicals, peptides, and recombinant proteins AG RNAex Pro Reagent Accurate Biology Cat#AG21102 Recombinant Mouse IL-2 R&D Systems Cat#402-ML-020 Recombinant Human IL-2 R&D Systems Cat#202-IL-050 DAPT APExBIO Cat#A8200 hsBCL9Z474 This paper N/A DMSO SIGMA Cat#D2660-100ML Ficoll-PaqueTM cytiva Cat#17144002 Fixation/Perm Diluent eBioscience Cat#00-5223-56 Fixation/Permeabilization Concentrate eBioscience Cat#00-5123-43 Permeabilization Buffer (10X) eBioscience Cat#00-8333-56 IC Fixation Buffer eBioscience Cat#00-8222-49 Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) eBioscience Cat#00-4975-03 ICG-001 APExBIO Cat#A8217 PageRulerTM Plus Prestained Protein Ladder Thermo Fisher Scientific Cat#26619 Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Retronectin Takara Cat#T100A FuGENE® HD Transfection Reagent Promega Cat#E2312 (Continued on next page) 18 Cell Reports 44, 115708, May 27, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays EasySepTM Mouse NK Cell Isolation Kit STEMCELL Cat#19855 EasySepTM Human NK Cell Isolation Kit STEMCELL Cat# 17955 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biology Cat#AG11701 Evo M-MLV RT Premix for qPCR Accurate Biology Cat#AG11706 ChIP Assay Kit Beyotime Cat#P2078 PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat#23225 Cytotoxicity Assay Kit (CFSE, 7-AAD) Abcam Cat#ab270780 PAGE Gel Fast Preparation Kit Epizyme Biotech Cat#PG111 Dual-LumiTM Luciferase Reporter Gene Assay Kit Beyotime Biotechnology Cat#RG088S Cell Counting Kit-8 Meilunbio Cat#MA0218-2 Deposited data scRNA-seq data This paper https://doi.org/10.6084/m9.figshare.

    Article Title: Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance.
    Article Snippet: In brief Classic tumor cell apoptosis and pyroptosis induced by tumor-specific T cells may not trigger sufficient primary responses and prevent tumor antigen escape-mediated acquired resistance.. Zheng et al. uncover a special tumor cell death induced by CAR-T, autosis, which has not been attributed to any T cell killingmechanism before but canmediate a potent type of bystander killing for antigen-negative tumor cells.

    Reporter Assay:

    Article Title: GATA1-mediated Notch signaling augment antitumor immunity of CD11b + CD27 - natural killer cells maturation via BCL9/β-catenin signal.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse-CD45-PerCP/Cy5.5 Invitrogen Cat#45-0451-82; RRID:AB_1107002 Anti-mouse-CD3e-eFluor 450 Invitrogen Cat#48-0031-80; RRID:AB_10733280 Anti-human/mouse-Granzyme B-Alexa Fluor 700 Biolegend Cat#372222; RRID:AB_2728389 Anti-mouse-Granzyme B-eFluor 450 eBioscience Cat#48-8898-82; RRID:AB_11149362 Anti-mouse-CD335 (NKp46)-Brilliant Violet 605 Biolegend Cat#137619; RRID:AB_2562452 Anti-mouse-CD335 (NKp46)-FITC eBioscience Cat#11-3351-82; RRID:AB_1210843 Anti-mouse-CD314 (NKG2D)-Alexa Fluor 488 eBioscience Cat#53-5882-82; RRID:AB_2811867 Anti-mouse-NK1.1-APC BioLegend Cat#108709; RRID:AB_313396 Anti-mouse-CD27-PE Biolegend Cat#124210; RRID:AB_1236459 Anti-mouse-CD11b-PE/Cy7 Biolegend Cat#10216 Anti-human-CD16-PE BioLegend Cat#360704; RRID:AB_2562749 Anti-human-CD56-APC BioLegend Cat#362504; RRID:AB_2563913 Anti-mouse-Ki-67-PE eBioscience Cat#12-5698-80; RRID:AB_11149672 Anti-human -CD3e-FITC MULTISCIENCES Cat#AH00301-100; RRID:AB_2890114 Anti-mouse -CD3e-FITC MULTISCIENCES Cat#AM003E0201-250 NOTCH1 Polyclonal antibody Proteintech Cat#20687-1-AP; RRID:AB_10700012 β-Actin Proteintech Cat#66009-1-Ig; RRID:AB_2687938 Bacterial and virus strains HBAAV2/9-ZsGreen Hanbio Cat#75010302 HBAAV2/9-Klrb1c-m-cre-3xflag-ZsGreen Hanbio Cat#81071807 Cignal Reporter Assay Kits QIAGEN Cat# CCS018L Chemicals, peptides, and recombinant proteins AG RNAex Pro Reagent Accurate Biology Cat#AG21102 Recombinant Mouse IL-2 R&D Systems Cat#402-ML-020 Recombinant Human IL-2 R&D Systems Cat#202-IL-050 DAPT APExBIO Cat#A8200 hsBCL9Z474 This paper N/A DMSO SIGMA Cat#D2660-100ML Ficoll-PaqueTM cytiva Cat#17144002 Fixation/Perm Diluent eBioscience Cat#00-5223-56 Fixation/Permeabilization Concentrate eBioscience Cat#00-5123-43 Permeabilization Buffer (10X) eBioscience Cat#00-8333-56 IC Fixation Buffer eBioscience Cat#00-8222-49 Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) eBioscience Cat#00-4975-03 ICG-001 APExBIO Cat#A8217 PageRulerTM Plus Prestained Protein Ladder Thermo Fisher Scientific Cat#26619 Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Retronectin Takara Cat#T100A FuGENE® HD Transfection Reagent Promega Cat#E2312 (Continued on next page) 18 Cell Reports 44, 115708, May 27, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays EasySepTM Mouse NK Cell Isolation Kit STEMCELL Cat#19855 EasySepTM Human NK Cell Isolation Kit STEMCELL Cat# 17955 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biology Cat#AG11701 Evo M-MLV RT Premix for qPCR Accurate Biology Cat#AG11706 ChIP Assay Kit Beyotime Cat#P2078 PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat#23225 Cytotoxicity Assay Kit (CFSE, 7-AAD) Abcam Cat#ab270780 PAGE Gel Fast Preparation Kit Epizyme Biotech Cat#PG111 Dual-LumiTM Luciferase Reporter Gene Assay Kit Beyotime Biotechnology Cat#RG088S Cell Counting Kit-8 Meilunbio Cat#MA0218-2 Deposited data scRNA-seq data This paper https://doi.org/10.6084/m9.figshare.

    Cell Stimulation:

    Article Title: GATA1-mediated Notch signaling augment antitumor immunity of CD11b + CD27 - natural killer cells maturation via BCL9/β-catenin signal.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse-CD45-PerCP/Cy5.5 Invitrogen Cat#45-0451-82; RRID:AB_1107002 Anti-mouse-CD3e-eFluor 450 Invitrogen Cat#48-0031-80; RRID:AB_10733280 Anti-human/mouse-Granzyme B-Alexa Fluor 700 Biolegend Cat#372222; RRID:AB_2728389 Anti-mouse-Granzyme B-eFluor 450 eBioscience Cat#48-8898-82; RRID:AB_11149362 Anti-mouse-CD335 (NKp46)-Brilliant Violet 605 Biolegend Cat#137619; RRID:AB_2562452 Anti-mouse-CD335 (NKp46)-FITC eBioscience Cat#11-3351-82; RRID:AB_1210843 Anti-mouse-CD314 (NKG2D)-Alexa Fluor 488 eBioscience Cat#53-5882-82; RRID:AB_2811867 Anti-mouse-NK1.1-APC BioLegend Cat#108709; RRID:AB_313396 Anti-mouse-CD27-PE Biolegend Cat#124210; RRID:AB_1236459 Anti-mouse-CD11b-PE/Cy7 Biolegend Cat#10216 Anti-human-CD16-PE BioLegend Cat#360704; RRID:AB_2562749 Anti-human-CD56-APC BioLegend Cat#362504; RRID:AB_2563913 Anti-mouse-Ki-67-PE eBioscience Cat#12-5698-80; RRID:AB_11149672 Anti-human -CD3e-FITC MULTISCIENCES Cat#AH00301-100; RRID:AB_2890114 Anti-mouse -CD3e-FITC MULTISCIENCES Cat#AM003E0201-250 NOTCH1 Polyclonal antibody Proteintech Cat#20687-1-AP; RRID:AB_10700012 β-Actin Proteintech Cat#66009-1-Ig; RRID:AB_2687938 Bacterial and virus strains HBAAV2/9-ZsGreen Hanbio Cat#75010302 HBAAV2/9-Klrb1c-m-cre-3xflag-ZsGreen Hanbio Cat#81071807 Cignal Reporter Assay Kits QIAGEN Cat# CCS018L Chemicals, peptides, and recombinant proteins AG RNAex Pro Reagent Accurate Biology Cat#AG21102 Recombinant Mouse IL-2 R&D Systems Cat#402-ML-020 Recombinant Human IL-2 R&D Systems Cat#202-IL-050 DAPT APExBIO Cat#A8200 hsBCL9Z474 This paper N/A DMSO SIGMA Cat#D2660-100ML Ficoll-PaqueTM cytiva Cat#17144002 Fixation/Perm Diluent eBioscience Cat#00-5223-56 Fixation/Permeabilization Concentrate eBioscience Cat#00-5123-43 Permeabilization Buffer (10X) eBioscience Cat#00-8333-56 IC Fixation Buffer eBioscience Cat#00-8222-49 Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) eBioscience Cat#00-4975-03 ICG-001 APExBIO Cat#A8217 PageRulerTM Plus Prestained Protein Ladder Thermo Fisher Scientific Cat#26619 Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Retronectin Takara Cat#T100A FuGENE® HD Transfection Reagent Promega Cat#E2312 (Continued on next page) 18 Cell Reports 44, 115708, May 27, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays EasySepTM Mouse NK Cell Isolation Kit STEMCELL Cat#19855 EasySepTM Human NK Cell Isolation Kit STEMCELL Cat# 17955 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biology Cat#AG11701 Evo M-MLV RT Premix for qPCR Accurate Biology Cat#AG11706 ChIP Assay Kit Beyotime Cat#P2078 PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat#23225 Cytotoxicity Assay Kit (CFSE, 7-AAD) Abcam Cat#ab270780 PAGE Gel Fast Preparation Kit Epizyme Biotech Cat#PG111 Dual-LumiTM Luciferase Reporter Gene Assay Kit Beyotime Biotechnology Cat#RG088S Cell Counting Kit-8 Meilunbio Cat#MA0218-2 Deposited data scRNA-seq data This paper https://doi.org/10.6084/m9.figshare.

    Transfection:

    Article Title: GATA1-mediated Notch signaling augment antitumor immunity of CD11b + CD27 - natural killer cells maturation via BCL9/β-catenin signal.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse-CD45-PerCP/Cy5.5 Invitrogen Cat#45-0451-82; RRID:AB_1107002 Anti-mouse-CD3e-eFluor 450 Invitrogen Cat#48-0031-80; RRID:AB_10733280 Anti-human/mouse-Granzyme B-Alexa Fluor 700 Biolegend Cat#372222; RRID:AB_2728389 Anti-mouse-Granzyme B-eFluor 450 eBioscience Cat#48-8898-82; RRID:AB_11149362 Anti-mouse-CD335 (NKp46)-Brilliant Violet 605 Biolegend Cat#137619; RRID:AB_2562452 Anti-mouse-CD335 (NKp46)-FITC eBioscience Cat#11-3351-82; RRID:AB_1210843 Anti-mouse-CD314 (NKG2D)-Alexa Fluor 488 eBioscience Cat#53-5882-82; RRID:AB_2811867 Anti-mouse-NK1.1-APC BioLegend Cat#108709; RRID:AB_313396 Anti-mouse-CD27-PE Biolegend Cat#124210; RRID:AB_1236459 Anti-mouse-CD11b-PE/Cy7 Biolegend Cat#10216 Anti-human-CD16-PE BioLegend Cat#360704; RRID:AB_2562749 Anti-human-CD56-APC BioLegend Cat#362504; RRID:AB_2563913 Anti-mouse-Ki-67-PE eBioscience Cat#12-5698-80; RRID:AB_11149672 Anti-human -CD3e-FITC MULTISCIENCES Cat#AH00301-100; RRID:AB_2890114 Anti-mouse -CD3e-FITC MULTISCIENCES Cat#AM003E0201-250 NOTCH1 Polyclonal antibody Proteintech Cat#20687-1-AP; RRID:AB_10700012 β-Actin Proteintech Cat#66009-1-Ig; RRID:AB_2687938 Bacterial and virus strains HBAAV2/9-ZsGreen Hanbio Cat#75010302 HBAAV2/9-Klrb1c-m-cre-3xflag-ZsGreen Hanbio Cat#81071807 Cignal Reporter Assay Kits QIAGEN Cat# CCS018L Chemicals, peptides, and recombinant proteins AG RNAex Pro Reagent Accurate Biology Cat#AG21102 Recombinant Mouse IL-2 R&D Systems Cat#402-ML-020 Recombinant Human IL-2 R&D Systems Cat#202-IL-050 DAPT APExBIO Cat#A8200 hsBCL9Z474 This paper N/A DMSO SIGMA Cat#D2660-100ML Ficoll-PaqueTM cytiva Cat#17144002 Fixation/Perm Diluent eBioscience Cat#00-5223-56 Fixation/Permeabilization Concentrate eBioscience Cat#00-5123-43 Permeabilization Buffer (10X) eBioscience Cat#00-8333-56 IC Fixation Buffer eBioscience Cat#00-8222-49 Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) eBioscience Cat#00-4975-03 ICG-001 APExBIO Cat#A8217 PageRulerTM Plus Prestained Protein Ladder Thermo Fisher Scientific Cat#26619 Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 Retronectin Takara Cat#T100A FuGENE® HD Transfection Reagent Promega Cat#E2312 (Continued on next page) 18 Cell Reports 44, 115708, May 27, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays EasySepTM Mouse NK Cell Isolation Kit STEMCELL Cat#19855 EasySepTM Human NK Cell Isolation Kit STEMCELL Cat# 17955 SYBR Green Premix Pro Taq HS qPCR Kit Accurate Biology Cat#AG11701 Evo M-MLV RT Premix for qPCR Accurate Biology Cat#AG11706 ChIP Assay Kit Beyotime Cat#P2078 PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat#23225 Cytotoxicity Assay Kit (CFSE, 7-AAD) Abcam Cat#ab270780 PAGE Gel Fast Preparation Kit Epizyme Biotech Cat#PG111 Dual-LumiTM Luciferase Reporter Gene Assay Kit Beyotime Biotechnology Cat#RG088S Cell Counting Kit-8 Meilunbio Cat#MA0218-2 Deposited data scRNA-seq data This paper https://doi.org/10.6084/m9.figshare.

    Recombinase Polymerase Amplification:

    Article Title: A T-cell-based immunogenicity protocol for evaluating human antigen-specific responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC mouse anti-human CD3 BioLegend Clone: SK7, Cat# 344803 APC mouse anti-human CD8 BioLegend Clone: RPA-T8, Cat# 301049 Brilliant Violet 785 mouse anti-human CD4 BioLegend Clone: RPA-T4, Cat# 300554 PE mouse anti-human IFN-g BioLegend Clone: B27, Cat# 506507 PE/Cyanine7 mouse anti-human TNF-a BioLegend Clone: Mab11, Cat# 502930 Brilliant Violet 605 rat anti-human IL-2 BioLegend Clone: MQ1-17H12, Cat# 500332 Purified mouse anti-human CD28 BD Biosciences Clone: CD28.2, Cat# 555726 Purified mouse anti-human CD49d BD Biosciences Clone: 9F10, Cat# 555502 Biological samples Healthy human peripheral blood mononuclear cells New York Blood Center https://nybloodcenter.org/ Chemicals, peptides, and recombinant proteins Custom peptides GenScript https://www.genscript.com/ peptide.html PepMix Human (MOG) JPT PM-MOG CEFT pool JPT PM-CEFT-3 (Continued on next page) 2 STAR Protocols 2, 100758, September 17, 2021 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Human granulocyte-macrophage colony-stimulating factor (GM-CSF) (LEUKINE (sargramostim)) Sanofi NDC 0024-5843-05 Human IL-4 R&D Systems Cat# 204-IL-010 Human Flt-3 Ligand R&D Systems Cat# 308-FKN-025 R848 InvivoGen Cat# tlrl-r848-5 Ultrapure LPS, Salmonella minnesota InvivoGen Cat# tlrl-smlps Human IL-1 beta R&D Systems Cat# 201-LB-010 Human IL-2 R&D Systems Cat# 202-IL-050 Human IL-7 R&D Systems Cat# 207-IL-025 Human IL-15 PeproTech Cat# 200-15 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# 79346-1MG Ionomycin calcium salt Sigma-Aldrich Cat# I0634-1MG Software and algorithms FACSDiva BD Biosciences N/A FlowJo v10.7.1 Tree Star www.flowjo.com/ Prism v9.1.0 GraphPad www.graphpad.com/ Other UltraComp eBead Plus Compensation Beads Thermo Fischer Scientific Cat# 01-3333-41 ArC Amine Reactive Compensation Bead Kit Thermo Fischer Scientific Cat# A10346 LIVE/DEAD Fixable Blue Dead Cell Stain Kit Thermo Fischer Scientific Cat# L34962 Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 GolgiStop: Protein Transport Inhibitor (containing Monensin) BD Biosciences Cat# 554724 GolgiPlug: Protein Transport Inhibitor (containing Brefeldin A) BD Biosciences Cat# 555029 Deoxyribonuclease I from bovine pancreas Sigma-Aldrich Cat# D4513-1VL DPBS, calcium, magnesium Gibco Cat# 14040133 X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza Cat# 04-418Q RPMI 1640 Medium Gibco Cat# 11875-093 Heat inactivated human serum Gemini Bio-Products Cat# 100-512 Nalgene Rapid-Flow Sterile Single Use Vacuum Filter Units Thermo Fischer Scientific Cat# 566-0020 96-well Clear Round Bottom TC-treated Microplate Corning Cat# 3799 ll OPEN ACCESSProtocol ..

    Purification:

    Article Title: A T-cell-based immunogenicity protocol for evaluating human antigen-specific responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC mouse anti-human CD3 BioLegend Clone: SK7, Cat# 344803 APC mouse anti-human CD8 BioLegend Clone: RPA-T8, Cat# 301049 Brilliant Violet 785 mouse anti-human CD4 BioLegend Clone: RPA-T4, Cat# 300554 PE mouse anti-human IFN-g BioLegend Clone: B27, Cat# 506507 PE/Cyanine7 mouse anti-human TNF-a BioLegend Clone: Mab11, Cat# 502930 Brilliant Violet 605 rat anti-human IL-2 BioLegend Clone: MQ1-17H12, Cat# 500332 Purified mouse anti-human CD28 BD Biosciences Clone: CD28.2, Cat# 555726 Purified mouse anti-human CD49d BD Biosciences Clone: 9F10, Cat# 555502 Biological samples Healthy human peripheral blood mononuclear cells New York Blood Center https://nybloodcenter.org/ Chemicals, peptides, and recombinant proteins Custom peptides GenScript https://www.genscript.com/ peptide.html PepMix Human (MOG) JPT PM-MOG CEFT pool JPT PM-CEFT-3 (Continued on next page) 2 STAR Protocols 2, 100758, September 17, 2021 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Human granulocyte-macrophage colony-stimulating factor (GM-CSF) (LEUKINE (sargramostim)) Sanofi NDC 0024-5843-05 Human IL-4 R&D Systems Cat# 204-IL-010 Human Flt-3 Ligand R&D Systems Cat# 308-FKN-025 R848 InvivoGen Cat# tlrl-r848-5 Ultrapure LPS, Salmonella minnesota InvivoGen Cat# tlrl-smlps Human IL-1 beta R&D Systems Cat# 201-LB-010 Human IL-2 R&D Systems Cat# 202-IL-050 Human IL-7 R&D Systems Cat# 207-IL-025 Human IL-15 PeproTech Cat# 200-15 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# 79346-1MG Ionomycin calcium salt Sigma-Aldrich Cat# I0634-1MG Software and algorithms FACSDiva BD Biosciences N/A FlowJo v10.7.1 Tree Star www.flowjo.com/ Prism v9.1.0 GraphPad www.graphpad.com/ Other UltraComp eBead Plus Compensation Beads Thermo Fischer Scientific Cat# 01-3333-41 ArC Amine Reactive Compensation Bead Kit Thermo Fischer Scientific Cat# A10346 LIVE/DEAD Fixable Blue Dead Cell Stain Kit Thermo Fischer Scientific Cat# L34962 Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 GolgiStop: Protein Transport Inhibitor (containing Monensin) BD Biosciences Cat# 554724 GolgiPlug: Protein Transport Inhibitor (containing Brefeldin A) BD Biosciences Cat# 555029 Deoxyribonuclease I from bovine pancreas Sigma-Aldrich Cat# D4513-1VL DPBS, calcium, magnesium Gibco Cat# 14040133 X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza Cat# 04-418Q RPMI 1640 Medium Gibco Cat# 11875-093 Heat inactivated human serum Gemini Bio-Products Cat# 100-512 Nalgene Rapid-Flow Sterile Single Use Vacuum Filter Units Thermo Fischer Scientific Cat# 566-0020 96-well Clear Round Bottom TC-treated Microplate Corning Cat# 3799 ll OPEN ACCESSProtocol ..

    Software:

    Article Title: A T-cell-based immunogenicity protocol for evaluating human antigen-specific responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC mouse anti-human CD3 BioLegend Clone: SK7, Cat# 344803 APC mouse anti-human CD8 BioLegend Clone: RPA-T8, Cat# 301049 Brilliant Violet 785 mouse anti-human CD4 BioLegend Clone: RPA-T4, Cat# 300554 PE mouse anti-human IFN-g BioLegend Clone: B27, Cat# 506507 PE/Cyanine7 mouse anti-human TNF-a BioLegend Clone: Mab11, Cat# 502930 Brilliant Violet 605 rat anti-human IL-2 BioLegend Clone: MQ1-17H12, Cat# 500332 Purified mouse anti-human CD28 BD Biosciences Clone: CD28.2, Cat# 555726 Purified mouse anti-human CD49d BD Biosciences Clone: 9F10, Cat# 555502 Biological samples Healthy human peripheral blood mononuclear cells New York Blood Center https://nybloodcenter.org/ Chemicals, peptides, and recombinant proteins Custom peptides GenScript https://www.genscript.com/ peptide.html PepMix Human (MOG) JPT PM-MOG CEFT pool JPT PM-CEFT-3 (Continued on next page) 2 STAR Protocols 2, 100758, September 17, 2021 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Human granulocyte-macrophage colony-stimulating factor (GM-CSF) (LEUKINE (sargramostim)) Sanofi NDC 0024-5843-05 Human IL-4 R&D Systems Cat# 204-IL-010 Human Flt-3 Ligand R&D Systems Cat# 308-FKN-025 R848 InvivoGen Cat# tlrl-r848-5 Ultrapure LPS, Salmonella minnesota InvivoGen Cat# tlrl-smlps Human IL-1 beta R&D Systems Cat# 201-LB-010 Human IL-2 R&D Systems Cat# 202-IL-050 Human IL-7 R&D Systems Cat# 207-IL-025 Human IL-15 PeproTech Cat# 200-15 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# 79346-1MG Ionomycin calcium salt Sigma-Aldrich Cat# I0634-1MG Software and algorithms FACSDiva BD Biosciences N/A FlowJo v10.7.1 Tree Star www.flowjo.com/ Prism v9.1.0 GraphPad www.graphpad.com/ Other UltraComp eBead Plus Compensation Beads Thermo Fischer Scientific Cat# 01-3333-41 ArC Amine Reactive Compensation Bead Kit Thermo Fischer Scientific Cat# A10346 LIVE/DEAD Fixable Blue Dead Cell Stain Kit Thermo Fischer Scientific Cat# L34962 Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 GolgiStop: Protein Transport Inhibitor (containing Monensin) BD Biosciences Cat# 554724 GolgiPlug: Protein Transport Inhibitor (containing Brefeldin A) BD Biosciences Cat# 555029 Deoxyribonuclease I from bovine pancreas Sigma-Aldrich Cat# D4513-1VL DPBS, calcium, magnesium Gibco Cat# 14040133 X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza Cat# 04-418Q RPMI 1640 Medium Gibco Cat# 11875-093 Heat inactivated human serum Gemini Bio-Products Cat# 100-512 Nalgene Rapid-Flow Sterile Single Use Vacuum Filter Units Thermo Fischer Scientific Cat# 566-0020 96-well Clear Round Bottom TC-treated Microplate Corning Cat# 3799 ll OPEN ACCESSProtocol ..

    Staining:

    Article Title: A T-cell-based immunogenicity protocol for evaluating human antigen-specific responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC mouse anti-human CD3 BioLegend Clone: SK7, Cat# 344803 APC mouse anti-human CD8 BioLegend Clone: RPA-T8, Cat# 301049 Brilliant Violet 785 mouse anti-human CD4 BioLegend Clone: RPA-T4, Cat# 300554 PE mouse anti-human IFN-g BioLegend Clone: B27, Cat# 506507 PE/Cyanine7 mouse anti-human TNF-a BioLegend Clone: Mab11, Cat# 502930 Brilliant Violet 605 rat anti-human IL-2 BioLegend Clone: MQ1-17H12, Cat# 500332 Purified mouse anti-human CD28 BD Biosciences Clone: CD28.2, Cat# 555726 Purified mouse anti-human CD49d BD Biosciences Clone: 9F10, Cat# 555502 Biological samples Healthy human peripheral blood mononuclear cells New York Blood Center https://nybloodcenter.org/ Chemicals, peptides, and recombinant proteins Custom peptides GenScript https://www.genscript.com/ peptide.html PepMix Human (MOG) JPT PM-MOG CEFT pool JPT PM-CEFT-3 (Continued on next page) 2 STAR Protocols 2, 100758, September 17, 2021 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Human granulocyte-macrophage colony-stimulating factor (GM-CSF) (LEUKINE (sargramostim)) Sanofi NDC 0024-5843-05 Human IL-4 R&D Systems Cat# 204-IL-010 Human Flt-3 Ligand R&D Systems Cat# 308-FKN-025 R848 InvivoGen Cat# tlrl-r848-5 Ultrapure LPS, Salmonella minnesota InvivoGen Cat# tlrl-smlps Human IL-1 beta R&D Systems Cat# 201-LB-010 Human IL-2 R&D Systems Cat# 202-IL-050 Human IL-7 R&D Systems Cat# 207-IL-025 Human IL-15 PeproTech Cat# 200-15 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# 79346-1MG Ionomycin calcium salt Sigma-Aldrich Cat# I0634-1MG Software and algorithms FACSDiva BD Biosciences N/A FlowJo v10.7.1 Tree Star www.flowjo.com/ Prism v9.1.0 GraphPad www.graphpad.com/ Other UltraComp eBead Plus Compensation Beads Thermo Fischer Scientific Cat# 01-3333-41 ArC Amine Reactive Compensation Bead Kit Thermo Fischer Scientific Cat# A10346 LIVE/DEAD Fixable Blue Dead Cell Stain Kit Thermo Fischer Scientific Cat# L34962 Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 GolgiStop: Protein Transport Inhibitor (containing Monensin) BD Biosciences Cat# 554724 GolgiPlug: Protein Transport Inhibitor (containing Brefeldin A) BD Biosciences Cat# 555029 Deoxyribonuclease I from bovine pancreas Sigma-Aldrich Cat# D4513-1VL DPBS, calcium, magnesium Gibco Cat# 14040133 X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza Cat# 04-418Q RPMI 1640 Medium Gibco Cat# 11875-093 Heat inactivated human serum Gemini Bio-Products Cat# 100-512 Nalgene Rapid-Flow Sterile Single Use Vacuum Filter Units Thermo Fischer Scientific Cat# 566-0020 96-well Clear Round Bottom TC-treated Microplate Corning Cat# 3799 ll OPEN ACCESSProtocol ..

    Article Title: Phocaeicola vulgatus induces immunotherapy resistance in hepatocellular carcinoma via reducing indoleacetic acid production.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Mouse IL-2 Recombinant Protein PeproTech Cat#212-12 Recombinant Human IL-2 R&D systems Cat202-IL-010 Human T cell TransAct Miltenyi Cat#130-111-160 Neomycin Sigma-Aldrich Cat#N5285-25G Metronidazole Sigma-Aldrich Cat#M1547-25G Vancomycin Sigma-Aldrich Cat#V8138-1G Ampicillin Sigma-Aldrich Cat#A1593-25G Indole-3-Acetic Acid Sodium Salt Sigma-Aldrich Cat#I5148 Dulbecco’s Modified Eagle Medium Gibco Cat#C11995500BT RPMI 1640 Medium Gibco Cat#C11875500BT PBS Gibco Cat#C20012500BT TRYPSIN 0.25% EDTA Gibco Cat#25200072 Fetal Bovine Serum Thermo Fisher Cat#10270-106 Penicillin-Streptomycin Gibco Cat#15140-122 TexMAC GMP Medium Miltenyi Cat#170-076-309 2-Mercaptoethanol Gibico Cat#21985023 D-Luciferin PerkinElmer Cat#122799 DNase I Roche Cat#10104159001 Liberase TM Research Grade Roche Cat#05401119001 Percoll GE Healthcare Cat#17-0891-01 RBC Lysis Buffer BioLegend Cat#420301 Cell Staining Buffer BioLegend Cat#420201 Leukocyte Activation Cocktail BD Biosciences Cat#550583 Protein Transport Inhibitor BD Biosciences Cat#555029 Triton X-100 Thermo Fisher Cat#85111 DAPI Thermo Fisher Cat#S36938 BSA Sigma‒Aldrich Cat#A9418 L-Amino Acid Rodent Diet without Added Tryptohan SYSE Bio-Tec. .. Co., Ltd. Cat#A22071501 L-Amino Acid Standard Rodent Diet SYSE Bio-Tec.

    Sterility:

    Article Title: A T-cell-based immunogenicity protocol for evaluating human antigen-specific responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC mouse anti-human CD3 BioLegend Clone: SK7, Cat# 344803 APC mouse anti-human CD8 BioLegend Clone: RPA-T8, Cat# 301049 Brilliant Violet 785 mouse anti-human CD4 BioLegend Clone: RPA-T4, Cat# 300554 PE mouse anti-human IFN-g BioLegend Clone: B27, Cat# 506507 PE/Cyanine7 mouse anti-human TNF-a BioLegend Clone: Mab11, Cat# 502930 Brilliant Violet 605 rat anti-human IL-2 BioLegend Clone: MQ1-17H12, Cat# 500332 Purified mouse anti-human CD28 BD Biosciences Clone: CD28.2, Cat# 555726 Purified mouse anti-human CD49d BD Biosciences Clone: 9F10, Cat# 555502 Biological samples Healthy human peripheral blood mononuclear cells New York Blood Center https://nybloodcenter.org/ Chemicals, peptides, and recombinant proteins Custom peptides GenScript https://www.genscript.com/ peptide.html PepMix Human (MOG) JPT PM-MOG CEFT pool JPT PM-CEFT-3 (Continued on next page) 2 STAR Protocols 2, 100758, September 17, 2021 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Human granulocyte-macrophage colony-stimulating factor (GM-CSF) (LEUKINE (sargramostim)) Sanofi NDC 0024-5843-05 Human IL-4 R&D Systems Cat# 204-IL-010 Human Flt-3 Ligand R&D Systems Cat# 308-FKN-025 R848 InvivoGen Cat# tlrl-r848-5 Ultrapure LPS, Salmonella minnesota InvivoGen Cat# tlrl-smlps Human IL-1 beta R&D Systems Cat# 201-LB-010 Human IL-2 R&D Systems Cat# 202-IL-050 Human IL-7 R&D Systems Cat# 207-IL-025 Human IL-15 PeproTech Cat# 200-15 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# 79346-1MG Ionomycin calcium salt Sigma-Aldrich Cat# I0634-1MG Software and algorithms FACSDiva BD Biosciences N/A FlowJo v10.7.1 Tree Star www.flowjo.com/ Prism v9.1.0 GraphPad www.graphpad.com/ Other UltraComp eBead Plus Compensation Beads Thermo Fischer Scientific Cat# 01-3333-41 ArC Amine Reactive Compensation Bead Kit Thermo Fischer Scientific Cat# A10346 LIVE/DEAD Fixable Blue Dead Cell Stain Kit Thermo Fischer Scientific Cat# L34962 Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 GolgiStop: Protein Transport Inhibitor (containing Monensin) BD Biosciences Cat# 554724 GolgiPlug: Protein Transport Inhibitor (containing Brefeldin A) BD Biosciences Cat# 555029 Deoxyribonuclease I from bovine pancreas Sigma-Aldrich Cat# D4513-1VL DPBS, calcium, magnesium Gibco Cat# 14040133 X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza Cat# 04-418Q RPMI 1640 Medium Gibco Cat# 11875-093 Heat inactivated human serum Gemini Bio-Products Cat# 100-512 Nalgene Rapid-Flow Sterile Single Use Vacuum Filter Units Thermo Fischer Scientific Cat# 566-0020 96-well Clear Round Bottom TC-treated Microplate Corning Cat# 3799 ll OPEN ACCESSProtocol ..

    Control:

    Article Title: Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance.
    Article Snippet: In brief Classic tumor cell apoptosis and pyroptosis induced by tumor-specific T cells may not trigger sufficient primary responses and prevent tumor antigen escape-mediated acquired resistance.. Zheng et al. uncover a special tumor cell death induced by CAR-T, autosis, which has not been attributed to any T cell killingmechanism before but canmediate a potent type of bystander killing for antigen-negative tumor cells.

    Immunopeptidomics:

    Article Title: Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance.
    Article Snippet: In brief Classic tumor cell apoptosis and pyroptosis induced by tumor-specific T cells may not trigger sufficient primary responses and prevent tumor antigen escape-mediated acquired resistance.. Zheng et al. uncover a special tumor cell death induced by CAR-T, autosis, which has not been attributed to any T cell killingmechanism before but canmediate a potent type of bystander killing for antigen-negative tumor cells.

    Enzyme-linked Immunospot:

    Article Title: Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance.
    Article Snippet: In brief Classic tumor cell apoptosis and pyroptosis induced by tumor-specific T cells may not trigger sufficient primary responses and prevent tumor antigen escape-mediated acquired resistance.. Zheng et al. uncover a special tumor cell death induced by CAR-T, autosis, which has not been attributed to any T cell killingmechanism before but canmediate a potent type of bystander killing for antigen-negative tumor cells.

    Activity Assay:

    Article Title: Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance.
    Article Snippet: In brief Classic tumor cell apoptosis and pyroptosis induced by tumor-specific T cells may not trigger sufficient primary responses and prevent tumor antigen escape-mediated acquired resistance.. Zheng et al. uncover a special tumor cell death induced by CAR-T, autosis, which has not been attributed to any T cell killingmechanism before but canmediate a potent type of bystander killing for antigen-negative tumor cells.

    Cell Isolation:

    Article Title: Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance.
    Article Snippet: In brief Classic tumor cell apoptosis and pyroptosis induced by tumor-specific T cells may not trigger sufficient primary responses and prevent tumor antigen escape-mediated acquired resistance.. Zheng et al. uncover a special tumor cell death induced by CAR-T, autosis, which has not been attributed to any T cell killingmechanism before but canmediate a potent type of bystander killing for antigen-negative tumor cells.

    RNA Sequencing:

    Article Title: Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance.
    Article Snippet: In brief Classic tumor cell apoptosis and pyroptosis induced by tumor-specific T cells may not trigger sufficient primary responses and prevent tumor antigen escape-mediated acquired resistance.. Zheng et al. uncover a special tumor cell death induced by CAR-T, autosis, which has not been attributed to any T cell killingmechanism before but canmediate a potent type of bystander killing for antigen-negative tumor cells.

    Gene Expression:

    Article Title: Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance.
    Article Snippet: In brief Classic tumor cell apoptosis and pyroptosis induced by tumor-specific T cells may not trigger sufficient primary responses and prevent tumor antigen escape-mediated acquired resistance.. Zheng et al. uncover a special tumor cell death induced by CAR-T, autosis, which has not been attributed to any T cell killingmechanism before but canmediate a potent type of bystander killing for antigen-negative tumor cells.

    Modification:

    Article Title: Phocaeicola vulgatus induces immunotherapy resistance in hepatocellular carcinoma via reducing indoleacetic acid production.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Mouse IL-2 Recombinant Protein PeproTech Cat#212-12 Recombinant Human IL-2 R&D systems Cat202-IL-010 Human T cell TransAct Miltenyi Cat#130-111-160 Neomycin Sigma-Aldrich Cat#N5285-25G Metronidazole Sigma-Aldrich Cat#M1547-25G Vancomycin Sigma-Aldrich Cat#V8138-1G Ampicillin Sigma-Aldrich Cat#A1593-25G Indole-3-Acetic Acid Sodium Salt Sigma-Aldrich Cat#I5148 Dulbecco’s Modified Eagle Medium Gibco Cat#C11995500BT RPMI 1640 Medium Gibco Cat#C11875500BT PBS Gibco Cat#C20012500BT TRYPSIN 0.25% EDTA Gibco Cat#25200072 Fetal Bovine Serum Thermo Fisher Cat#10270-106 Penicillin-Streptomycin Gibco Cat#15140-122 TexMAC GMP Medium Miltenyi Cat#170-076-309 2-Mercaptoethanol Gibico Cat#21985023 D-Luciferin PerkinElmer Cat#122799 DNase I Roche Cat#10104159001 Liberase TM Research Grade Roche Cat#05401119001 Percoll GE Healthcare Cat#17-0891-01 RBC Lysis Buffer BioLegend Cat#420301 Cell Staining Buffer BioLegend Cat#420201 Leukocyte Activation Cocktail BD Biosciences Cat#550583 Protein Transport Inhibitor BD Biosciences Cat#555029 Triton X-100 Thermo Fisher Cat#85111 DAPI Thermo Fisher Cat#S36938 BSA Sigma‒Aldrich Cat#A9418 L-Amino Acid Rodent Diet without Added Tryptohan SYSE Bio-Tec. .. Co., Ltd. Cat#A22071501 L-Amino Acid Standard Rodent Diet SYSE Bio-Tec.

    Red Blood Cell Lysis:

    Article Title: Phocaeicola vulgatus induces immunotherapy resistance in hepatocellular carcinoma via reducing indoleacetic acid production.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Mouse IL-2 Recombinant Protein PeproTech Cat#212-12 Recombinant Human IL-2 R&D systems Cat202-IL-010 Human T cell TransAct Miltenyi Cat#130-111-160 Neomycin Sigma-Aldrich Cat#N5285-25G Metronidazole Sigma-Aldrich Cat#M1547-25G Vancomycin Sigma-Aldrich Cat#V8138-1G Ampicillin Sigma-Aldrich Cat#A1593-25G Indole-3-Acetic Acid Sodium Salt Sigma-Aldrich Cat#I5148 Dulbecco’s Modified Eagle Medium Gibco Cat#C11995500BT RPMI 1640 Medium Gibco Cat#C11875500BT PBS Gibco Cat#C20012500BT TRYPSIN 0.25% EDTA Gibco Cat#25200072 Fetal Bovine Serum Thermo Fisher Cat#10270-106 Penicillin-Streptomycin Gibco Cat#15140-122 TexMAC GMP Medium Miltenyi Cat#170-076-309 2-Mercaptoethanol Gibico Cat#21985023 D-Luciferin PerkinElmer Cat#122799 DNase I Roche Cat#10104159001 Liberase TM Research Grade Roche Cat#05401119001 Percoll GE Healthcare Cat#17-0891-01 RBC Lysis Buffer BioLegend Cat#420301 Cell Staining Buffer BioLegend Cat#420201 Leukocyte Activation Cocktail BD Biosciences Cat#550583 Protein Transport Inhibitor BD Biosciences Cat#555029 Triton X-100 Thermo Fisher Cat#85111 DAPI Thermo Fisher Cat#S36938 BSA Sigma‒Aldrich Cat#A9418 L-Amino Acid Rodent Diet without Added Tryptohan SYSE Bio-Tec. .. Co., Ltd. Cat#A22071501 L-Amino Acid Standard Rodent Diet SYSE Bio-Tec.

    Activation Assay:

    Article Title: Phocaeicola vulgatus induces immunotherapy resistance in hepatocellular carcinoma via reducing indoleacetic acid production.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Mouse IL-2 Recombinant Protein PeproTech Cat#212-12 Recombinant Human IL-2 R&D systems Cat202-IL-010 Human T cell TransAct Miltenyi Cat#130-111-160 Neomycin Sigma-Aldrich Cat#N5285-25G Metronidazole Sigma-Aldrich Cat#M1547-25G Vancomycin Sigma-Aldrich Cat#V8138-1G Ampicillin Sigma-Aldrich Cat#A1593-25G Indole-3-Acetic Acid Sodium Salt Sigma-Aldrich Cat#I5148 Dulbecco’s Modified Eagle Medium Gibco Cat#C11995500BT RPMI 1640 Medium Gibco Cat#C11875500BT PBS Gibco Cat#C20012500BT TRYPSIN 0.25% EDTA Gibco Cat#25200072 Fetal Bovine Serum Thermo Fisher Cat#10270-106 Penicillin-Streptomycin Gibco Cat#15140-122 TexMAC GMP Medium Miltenyi Cat#170-076-309 2-Mercaptoethanol Gibico Cat#21985023 D-Luciferin PerkinElmer Cat#122799 DNase I Roche Cat#10104159001 Liberase TM Research Grade Roche Cat#05401119001 Percoll GE Healthcare Cat#17-0891-01 RBC Lysis Buffer BioLegend Cat#420301 Cell Staining Buffer BioLegend Cat#420201 Leukocyte Activation Cocktail BD Biosciences Cat#550583 Protein Transport Inhibitor BD Biosciences Cat#555029 Triton X-100 Thermo Fisher Cat#85111 DAPI Thermo Fisher Cat#S36938 BSA Sigma‒Aldrich Cat#A9418 L-Amino Acid Rodent Diet without Added Tryptohan SYSE Bio-Tec. .. Co., Ltd. Cat#A22071501 L-Amino Acid Standard Rodent Diet SYSE Bio-Tec.



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